Journal: bioRxiv
Article Title: The miR-183/96/182 Cluster Regulates Trigeminal Ganglion Sensory Neurons’ Response to Pseudomonas aeruginosa Infection
doi: 10.64898/2026.03.30.715374
Figure Lengend Snippet: ELISA assays in the supernatant of the axon chambers. NTC: no-treatment control; a-TLR+PA: the axon chamber pretreated with TLR4-specific antagonist, LPS-RS Ultrapure (20 µg/mL) 2 hours before PA infection; a-Fpr+PA: the axon chamber pre-treated with Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection; a-TLR/Fpr+PA: the axon chamber pre-treated with both LPS-RS Ultrapure (20 µg/mL) and Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection. *: p<0.05, **: p<0.01, ***:p<0.001 by one-way ANOVA of multiple sample comparison with Bonferroni post hoc test. #: p<0.05, ##: p<0.01, ###: p<0.001 by Student’s t test.
Article Snippet: To test this hypothesis, we pre-treated the neurites in the axon chambers with a TLR4-specific antagonist, TLR4-RS (InVivoGen), and/or a FPR1-specific antagonist, Boc-MLF (TOCRIS) 2 hours before PA infection.
Techniques: Enzyme-linked Immunosorbent Assay, Control, Infection, Comparison